Expertise and Knowledge in the Age of Personalized Media: The Case of @anysports.faceonline Blog in the Period from 2018 to 2019

Digital age, that we are living in, enables us to instantly access great volumes of various information. The way we acquire, create and distribute knowledge is a subject to continuous transformation caused by the rapid growth of digital content and tools. Nowadays, more and more people give their own contribution in digital knowledge environment by producing and sharing their digital content. This thesis considers the impact that digital culture has put on the way we consume and create knowledge and establish the image of trustworthy expert in a certain field. This research is a case study of the Instagram account @anysports.faceonline. It represents a qualitative research aiming to analyze visual rhetoric and knowledge representation in the @anysports.faceonline blog in order to examine the mechanics of impression management, concerning the establishment and gaining acknowledgement of professionalism / expertise within online environment. By visual rhetoric is meant, in the first place, a form of visual communication the influencer uses. It involves the visual structure, displaying of information, color usage and designing of self-representation. Moreover, this research is not constrained in cultural history only, it refers to other fields such as psychology, sociology, marketing, media and gender studies. However, in order to explain the chosen visual strategies, the object of the research is, firstly, put into a broad cultural context

Portable potentiometric device for determining the antioxidant capacity

At present, the development of portable devices for the express assessment of the content of biologically active objects, such as antioxidants, is one of the relevant technological problems of modern chemistry, medicine, and engineering. The main advantages of such devices are the simplicity and rapidity of analysis, small volumes of analyte, as well as miniaturization of equipment, making it possible to carry out the on-site analysis and, thus, to take a step towards the personalized medicine. The potentiometric method using the K3[Fe(CN)6]/K4[Fe(CN)6] system, which in the laboratory-scale version proved to be the most accurate, reproducible, and express, was the basis for the developed prototypes of portable devices. In this study, two versions of prototypes of the portable device are proposed, namely, the open microcell with the 0.2 ml volume and the microfluidic device with flow control. The correctness of the antioxidant capacity (AOC) determination in both systems was confirmed by comparing the results of the "introduced-found" method on model solutions of antioxidants and their mixtures with the AOC results obtained in a standard laboratory electrochemical cell. The relative standard deviation did not exceed 10%. The AOC of some beverage industry was determined using the microfluidic device. The correlation coefficient of the results, obtained in the microfluidic device and the laboratory cell, was 0.90, which indicates good data convergence and the possibility of using the potentiometric method implemented in the microfluidic device to assess the AOC of multicomponent objects

Abstract P-24: Microscopic Analyses of Liquid-Liquid Phase Separation Induced by Linker Histone H1.0

Background: Liquid-liquid phase separation (LLPS) that leads to the formation of temporary functional domains in cells plays an important role in the processes of chromatin condensation and gene regulation. Earlier, it was demonstrated that histone H1.4 can form LLPS droplets with DNA. In the present work, LLPS was studied for histone H1.0, which is mainly expressed in differentiated and non-dividing cells. H1.0 is involved in cancer development: its amount decreases with the progression of tumor cells to malignancy. Methods: LSM710 confocal microscope (Zeiss) equipped with the 40x/1.2W objective was used to image mixtures of H1.0 with Cy3/Cy5 labeled DNA or nucleosomes in fluorescent and transmitted-light channels at the excitation of 514 nm. The formation of condensates as a result of LLPS was confirmed by salt-jump and FRAP/FLIP experiments. Results: Condensates were not observed when the ratio of negative to positive charges (N/P) in the samples was >1. At N/P~0.7, optically homogeneous droplet-like condensates were found. The appearance of condensates, their size and shape depended on concentrations of H1.0 and DNA. LLPS condensates but not aggregates disappeared by salt-jump to 650 mM NaCl. FRAP/FLIP experiments revealed a moderate rate of fluorescence recovery (τ½22s) indicating moderate DNA mobility of the H1.0-mediated condensates. The appearance of condensates was also observed in the mixtures of H1.0, DNA and Cy3/Cy5-labeled nucleosomes. Nucleosomes were involved in the condensate formation and found to be 2-fold more mobile (τ½10 s) than DNA. Conclusion: LLPS-related properties of H1.0 were studied for DNA and nucleosomes in vitro. Comparison with H1.4 shows that H1.0 forms liquid condensates of approximately the same size. Our result also may indicate that chromatin retains pronounced dynamic properties in H1.0-induced droplets despite the fact that H1.0 induces the formation of more compact chromatin

ТЕОРЕТИКО-МЕТОДОЛОГИЧЕСКИЕ ОСНОВЫ СОЦИАЛЬНОЙ ЗАЩИТЫ НАСЕЛЕНИЯ В РОССИЙСКОЙ ИМПЕРИИ

Purpose: to ascertain theoretical and methodological bases and terminological apparatus of the notion social protection of the population in the Russian empire.Methodology: for the realization of the objective task which is to ascertain theoretical and methodological bases of the social protection of the population in the Russian empire, the basic methodological principles of objectivity and historicism have been applied.Results: the author supposes that the term used in pre-revolutionary Russia as «care, charity» and which meant care, «charge» of unprotected strata of the population by the society and the state is correlated with the modern idea of social protection of the population. Besides, stable notions, which were in pre-revolutionary lexicon, have been revealed: care of the poor, parish board of guardians, guardianship of public temperance, guardianship of prisons.Practical implications: the results may be used for examining the experience of formation, functioning and development of social protection of population, its organizing and coordinating in the regional systems. Also, the results may be used while developing generalized works devoted to the problems of the national history, text books as well as while developing university courses in the Russian history, history of state administration in Russia.Цель: выявить теоретико-методологические основы и терминологический аппарат понятия социальной защиты населения в Российской империи.Метод или методология проведения работы: для выполнения целевой задачи выявления теоретико-методологические основы социальной защиты населения в Российской империи, использовались базовые методологические принципы: объективность и историзм.Результаты: автор предполагает, что термин, используемый в дореволюционной России как «призрение» и означавший заботу, «попечение» общества и государства о незащищенных слоях населения соотносится с современным понятием социальной защиты населения. Кроме того, выявлены устойчивые понятия, находящиеся в дореволюционном лексиконе: попечительство о бедных, приходские попечительства, попечительство о народной трезвости, попечительство о тюрьмах.Область применения результатов: результаты могут быть использованы для изучения опыта становления, функционирования и развития социальной защиты населения, ее организации и координации в региональных системах. Также результаты могут быть использованы при разработке обобщающих работ, посвященных проблемам отечественной истории, учебных пособий, при подготовке вузовских курсов по отечественной истории, истории государственного управления в России

Transformation of the values of Generation Z - residents of the digital society of sustainable development

The transition to a society of sustainable development is possible only when the priorities and values of the active part of society are changing. From the values of individualism, the weak ties of corporatism, the extensive growth of an industrial society to the values of networked solidarity, social justice, the strong ties of the information/digital society. The transition to a new level of civilizational development not only contributed to the transformation of the economy, politics and the social sphere under the influence of digital technologies, but played a significant role in the formation of Generation Z (born in the late 20th and early 21st centuries). The article contains study material that was carried out at the Ufa State Petroleum Technological University in the summer of 2020

Macrophage Dysfunction in Autoimmune Rheumatic Diseases and Atherosclerosis

One of the problems of modern medical science is cardiovascular pathology caused by atherosclerotic vascular lesions in patients with autoimmune rheumatic diseases (ARDs). The similarity between the mechanisms of the immunopathogenesis of ARD and chronic low-grade inflammation in atherosclerosis draws attention. According to modern concepts, chronic inflammation associated with uncontrolled activation of both innate and acquired immunity plays a fundamental role in all stages of ARDs and atherosclerotic processes. Macrophage monocytes play an important role among the numerous immune cells and mediators involved in the immunopathogenesis of both ARDs and atherosclerosis. An imbalance between M1-like and M2-like macrophages is considered one of the causes of ARDs. The study of a key pathogenetic factor in the development of autoimmune and atherosclerotic inflammation-activated monocyte/macrophages will deepen the knowledge of chronic inflammation pathogenesis

Activation Markers on B and T Cells and Immune Checkpoints in Autoimmune Rheumatic Diseases

In addition to identifying the major B- and T-cell subpopulations involved in autoimmune rheumatic diseases (ARDs), in recent years special attention has been paid to studying the expression of their activation markers and immune checkpoints (ICPs). The activation markers on B and T cells are a consequence of the immune response, and these molecules are considered as sensitive specific markers of ARD activity and as promising targets for immunotherapy. ICPs regulate the activation of the immune response by preventing the initiation of autoimmune processes, and they modulate it by reducing immune cell-induced organ and tissue damage. The article considers the possible correlation of ICPs with the activity of ARDs, the efficacy of specific ARD treatments, and the prospects for the use of activation molecules and activation/blocking ICPs for the treatment of ARDs

Thinking in messages – determine key performance indicators and create environmental reports

Environmental reporting aims to measure, disclose and to be accountable to internal and external stakeholders about to what extent the company uses the functions of the environment and takes action to improve environmental performance. Thus, the communication of a company has two objectives: for internal stakeholders’ environmental performance is measured in order to prepare decisions of corporate management, for external stakeholders the reporting on the environmental management system, the environmental performance, the ecology orientation of the value-added steps, the dealing with stakeholders and process and product innovations as well as environmental objectives are in the foreground. Finally, it should be noted that environmental reporting is increasingly part of sustainability reporting

Systemic Sclerosis and Atherosclerosis: Potential Cellular Biomarkers and Mechanisms

Systemic sclerosis (SSc) is a rare systemic autoimmune disease of unknown etiology, which is characterized by endothelial dysfunction, pathologic vasculopathy, and increased tissue fibrosis. Traditionally, SSc has been regarded as a prototypical fibrotic disease in the family of systemic autoimmune diseases. Traditionally, emphasis has been placed on the three components of the pathogenesis of SSc: vascular, immune, and mesenchymal. Microvascular lesions, including endothelial dysfunction and smooth muscle cell migration into the intima of vessels in SSc, resemble the atherosclerotic process. Although microvascular disease is a hallmark of SSc, understanding the role of atherosclerotic vascular lesions in patients with SSc remains limited. It is still unknown whether the increased cardiovascular risk in SSc is related to specific cardiac complications (such as myocardial fibrosis) or the accelerated development of atherosclerosis. Different immune cell types appear to be involved in the immunopathogenesis of SSc via the activation of other immune cells, fibrosis, or vascular damage. Macrophages, B cells, T cells, dendritic cells, neutrophils, and endothelial cells have been reported to play the most important role in the pathogenesis of SSc and atherosclerosis. In our article, we reviewed the most significant and recent studies on the pathogenetic links between the development of SSc and the atherosclerotic process

Genetic Diversity and Primary Drug Resistance of Mycobacterium tuberculosis Beijing Genotype Strains in Northwestern Russia

The Beijing genotype is the main family of Mycobacterium tuberculosis in Russia. We analyzed its diversity and drug resistance in provinces across Northwestern Russia to identify the epidemiologically relevant Beijing strains. The study collection included 497 isolates from newly-diagnosed tuberculosis (TB) patients. Bacterial isolates were subjected to drug-susceptibility testing and genotyping. The Beijing genotype was detected in 57.5% (286/497); 50% of the Beijing strains were multidrug-resistant (MDR). Central Asian/Russian and B0/W148 groups included 176 and 77 isolates, respectively. MDR was more frequent among B0/W148 strains compared to Central Asian/Russian strains (85.7% vs. 40.3%, p < 0.0001). Typing of 24 minisatellite loci of Beijing strains revealed 82 profiles; 230 isolates were in 23 clusters. The largest Central Asian/Russian types were 94-32 (n = 75), 1065-32 (n = 17), and 95-32 (n = 12). B0/W148 types were 100-32 (n = 59) and 4737-32 (n = 5). MDR was more frequent in types 1065-32 (88.2%), 100-32 (83.1%), and 4737-32 (100%). In contrast, type 9391-32 (n = 9) included only drug-susceptible strains. To conclude, M. tuberculosis Beijing genotype is dominant in Northwestern Russia, and an active transmission of overwhelmingly MDR B0/W148 types explains the reported increase of MDR-TB. The presence of MDR-associated minor variants (type 1071-32/ancient Beijing and Central Asia Outbreak strain) in some of the studied provinces also requires attention